FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_4w-49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_4w-49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12320820
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC131080.10638902280854683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG26800.040.0301115
CTCGTAT22600.038.16094642
TCGTATG26800.036.81464443
TATGCCG26650.036.5045146
ACACGTC33100.033.45296513
CACGTCT32500.033.3277214
CGTATGC31400.031.20231644
CAGTCAC34050.029.07635727
TGTTACC33950.028.24636813
CTTAAAC29100.027.6275371
TTACCTC34900.027.28002515
GTATGCC36400.026.91606545
GTCGCTA30250.026.0006827
TCTAGTT44250.025.6475287
CTAATGC37550.025.44739321
ACCTATA31100.024.9570856
CGCTATG31150.024.91722529
CTAATCT35200.024.69656837
TCTAATG38700.024.69130320
GTCTGAA44200.024.50637617
Produced by FastQC (version 0.11.2)