FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_4w-45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_4w-45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12994563
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC132990.10234280290918595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG29550.040.5079215
CTCGTAT25550.040.5060644
TCGTATG27600.039.3721845
CACGTCT35050.035.03656814
GTCACAC31900.033.84789729
TATCTCG31900.032.65910341
CGTATGC33750.032.1978246
TATGCCG33500.032.1292948
CAGTCAC37650.029.50306927
ACACGTC42200.029.42724813
AGTCACA37250.029.35683828
TGTTACC34050.029.27792713
TCACACT39750.027.59749430
ACTGATA39450.027.5441834
TCTAGTT42100.026.8758017
TTACCTC38600.026.18474215
GTATGCC42500.025.731147
TAGTTGT39750.025.6869499
CACACGT49700.024.57007412
ATATCTC43350.024.27161840
Produced by FastQC (version 0.11.2)