FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_4w-44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_4w-44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10903682
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGAGACATCATCGAATCAGAGATGAAC174300.15985425840555512No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC168660.15468169376179533No Hit
GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAAT138690.12719556568139093No Hit
CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATT137090.12572817145621085No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGCATTCCTCGTTGAAG134790.12361879225751449No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC128460.11781341385414577No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC114870.10534973415402248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG26050.044.36170215
CTCGTAT21750.042.18941542
CACGTCT27850.041.86553614
CGTATGC25750.039.92345444
TCGTATG26150.039.4441543
TATGCCG28100.036.58432446
CAGTCAC32700.033.0200527
ACTCAAT33150.031.73901635
ACACGTC39600.029.79134613
TTACCTC33000.028.85135815
CGCTATG18600.028.5621529
TCTAGTT40550.027.9878927
AGTCACC38300.027.92183928
ATGCCGT37950.027.63457947
GTCTGAA43200.027.15027817
TCGGAAG42850.027.130653
CTCTAGT39500.027.0734586
GTCGCTA20050.026.3243827
ACTCTAG39650.026.1011225
TGTTACC37350.025.76753213