FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_4w-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_4w-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11014859
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC159290.14461374403430857No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG135070.12262526465386438No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG119650.10862599330595153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG21450.041.00903715
TCGTATG18500.038.2266843
CTCGTAT16050.037.82875442
CACGTCT24500.035.62158214
TATGCCG21050.034.90885546
CGTATGC21350.033.93310544
ACACGTC28050.030.9902113
CGCTATG25700.029.66454529
CTTAAAC28650.027.5796031
GTCGCTA28050.027.05617327
GTCTGAA33700.026.61399817
CAGTCAC30300.025.84400227
GTATGCC29150.025.20862445
GGTAACT29700.025.10121369
ACCTATA30250.025.0873136
TAAACCT33500.023.6838173
CTATAAG37000.023.1237446
TTAAACC34700.022.8650912
TATAAGC37900.022.75636947
ATGCCGT32250.022.46424147
Produced by FastQC (version 0.11.2)