FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_4s-59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_4s-59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14368132
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC203680.14175816313491552No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG178970.12456038126598502No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG158260.11014653818603559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATC24450.048.25242638
ACGTCTG33600.043.2229815
CTCGTAT27900.041.66925444
TACGTAA31500.041.0672536
ACGTAAT32750.040.65860437
TCGTATG31900.039.58097545
CCGTACG34100.038.23991433
CACGTCT37600.038.16566514
TATGCCG32900.038.06373248
ACACGTC40400.036.20272413
CGTATGC35750.035.51165446
CAGTCAC41650.031.55709527
CGCTATG28400.029.6388629
GTATGCC43000.029.5244347
TAATCTC42200.028.12075440
GTCGCTA30550.027.77863727
ACCGTAC48950.027.48491532
GTCTGAA53850.027.1619617
ATGCCGT46650.026.84507849
CTTAAAC34100.025.803591
Produced by FastQC (version 0.11.2)