FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_4s-53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_4s-53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15288358
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC198670.12994855301007474No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA180240.11789362860288854TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT36900.042.53687344
TCGTATG39650.041.3267245
GTCACGT40400.041.24262229
ACGTCTG44950.040.75046515
ACGTCCG41250.040.05721732
TCACGTC42750.038.49131830
TATGCCG42600.038.3033148
CGCACAT43700.037.2591237
CGTATGC45000.036.87327246
CCGCACA44650.036.6977936
CACGTCT51700.035.96336414
AGTCACG46550.035.86768728
ACACGTC52400.035.3508113
GTCCGCA49450.033.41432634
TGTTACC45750.030.98896613
GTATGCC54400.030.8824747
CAGTCAC55550.030.42924127
CACACGT61250.030.13034212
TCTAGTT53600.028.5746067
TTACCTC51450.027.42241515
Produced by FastQC (version 0.11.2)