FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_4s-51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_4s-51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12837013
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC144790.11279103635713386No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC142130.1107189032214893No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG128770.10031149769810156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT23000.043.64742744
ACGTCTG28300.042.41904415
GTCACGT25600.042.17767329
TCGTATG26500.040.2259345
TCACGTG30250.036.3786830
TATGCCG28750.035.87816248
AGTCACG30900.035.27823628
CACGTCT34200.034.9997314
TATCTCG29100.034.97189341
ACACGTC34900.034.49527713
CGTATGC31000.034.3866846
GCCTTAT32600.033.6503437
CAGTCAC36400.030.99033727
CGCTATG31900.030.92812529
GTCGCTA32300.030.54523327
CCTTATC34450.030.2413438
TGTTACC30500.030.08440813
CTTAAAC33250.029.5768261
GTATGCC36800.028.96692747
TTACCTC32400.028.53368615
Produced by FastQC (version 0.11.2)