FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_4s-50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_4s-50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13335811
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC188470.14132623805181402No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA164500.12335207810008704TruSeq Adapter, Index 19 (97% over 40bp)
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC156700.11750316497436863No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG147560.11064943856807809No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG143230.10740254192264723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT29150.046.03542744
TGAAACG32500.044.89763335
GTCACGT32550.044.61821729
TCGTATG32600.044.23232345
ACGTCTG36650.042.73151815
CACGTGA34350.042.38072631
TCACGTG35650.040.7382230
TATGCCG35800.040.278948
AGTCACG37100.038.7740928
GAAACGA37250.038.70910636
CGTATGC38050.038.2596146
CACGTCT42450.037.21726214
ACACGTC43550.036.43549713
GTATGCC42850.034.2150847
CAGTCAC43800.033.47297327
ATGCCGT46650.031.28069149
TGTTACC38250.030.8425113
GTCTGAA52450.030.25417317
TTACCTC41000.028.43801515
TCTAGTT47550.027.9303677