Basic Statistics
Measure | Value |
---|---|
Filename | HYJKMBGXY_n01_4s-50.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 13335811 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC | 18847 | 0.14132623805181402 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA | 16450 | 0.12335207810008704 | TruSeq Adapter, Index 19 (97% over 40bp) |
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC | 15670 | 0.11750316497436863 | No Hit |
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG | 14756 | 0.11064943856807809 | No Hit |
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG | 14323 | 0.10740254192264723 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 2915 | 0.0 | 46.035427 | 44 |
TGAAACG | 3250 | 0.0 | 44.897633 | 35 |
GTCACGT | 3255 | 0.0 | 44.618217 | 29 |
TCGTATG | 3260 | 0.0 | 44.232323 | 45 |
ACGTCTG | 3665 | 0.0 | 42.731518 | 15 |
CACGTGA | 3435 | 0.0 | 42.380726 | 31 |
TCACGTG | 3565 | 0.0 | 40.73822 | 30 |
TATGCCG | 3580 | 0.0 | 40.2789 | 48 |
AGTCACG | 3710 | 0.0 | 38.77409 | 28 |
GAAACGA | 3725 | 0.0 | 38.709106 | 36 |
CGTATGC | 3805 | 0.0 | 38.25961 | 46 |
CACGTCT | 4245 | 0.0 | 37.217262 | 14 |
ACACGTC | 4355 | 0.0 | 36.435497 | 13 |
GTATGCC | 4285 | 0.0 | 34.21508 | 47 |
CAGTCAC | 4380 | 0.0 | 33.472973 | 27 |
ATGCCGT | 4665 | 0.0 | 31.280691 | 49 |
TGTTACC | 3825 | 0.0 | 30.84251 | 13 |
GTCTGAA | 5245 | 0.0 | 30.254173 | 17 |
TTACCTC | 4100 | 0.0 | 28.438015 | 15 |
TCTAGTT | 4755 | 0.0 | 27.930367 | 7 |