FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_4s-49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_4s-49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12523880
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG169770.13555703184636073No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC150360.12005863997419329No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC136570.10904767532106663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG24150.047.70842433
ACGTTTC27050.042.72220632
GTCACGT27700.041.84423429
TTTCGGA27950.041.7164735
ACGTCTG29700.041.5810815
CACGTTT29550.039.57492431
TTCGGAA30250.038.6588336
ACACGTC34850.035.9298713
TCGGAAT32650.035.8171437
AGTCACG33050.035.17489628
CTCGTAT20450.035.08783344
CACGTCT36100.034.2084114
TCGTATG21550.032.65648345
CAGTCAC36850.031.54788627
CGCTATG27600.029.12211829
GTCTGAA44400.028.51418317
GTCGCTA28800.028.14836727
CTTAAAC26900.027.7072721
TGTTACC33300.027.13993813
TATGCCG24800.026.9867448
Produced by FastQC (version 0.11.2)