FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_4s-45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_4s-45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15364362
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC189700.1234675413141138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT37900.044.05351629
ACGTCTG42250.043.51775415
CTCGTAT37150.043.4574642
TATGCCG41450.040.36494446
TCGTATG42800.039.49411843
CGTATGC43300.038.4811344
TCACGTA44100.038.0949730
CACGTCT49950.037.01540814
AGTCACG46450.036.01878428
ACACGTC52350.035.77967513
CACGTAG48100.035.1419231
ACGTAGA48350.034.7458332
CGTAGAG51050.033.1106533
GTATGCC53050.031.79879245
CAGTCAC57450.030.08297227
CACACGT62800.029.5512512
GTCTGAA66400.028.46959117
ATCGGAA67250.027.2894742
TGTTACC44850.027.07281713
ATGCCGT64650.026.89352647
Produced by FastQC (version 0.11.2)