FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_4s-44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_4s-44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13143374
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC164920.12547767414972746No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG137470.10459262591173317No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA133090.10126014826938655TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT27450.041.47422444
ACGTCTG32400.039.71328415
TCGTATG30600.038.33223745
TATGCCG30950.038.12125448
CACGAGT30950.038.00819431
CACGTCT35900.036.03314214
CGTATGC32350.035.83191746
ACACGTC36450.035.7730813
TATCTCG32750.034.76183341
AGTCACG37200.031.80763628
ACGAGTG37400.031.63718432
TCACGAG38300.030.89422230
GTATGCC38400.030.54553447
CAGTCAC40800.029.67760527
CGCTATG29200.029.53525229
GTCACGA42250.028.00599129
GTCGCTA30450.027.86953427
TGTTACC35700.027.63502713
CTTAAAC34250.027.5043431
TTACCTC36500.026.93543215
Produced by FastQC (version 0.11.2)