FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_4s-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_4s-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14426472
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG225720.15646237000979865No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC196340.13609703051445982No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG164710.11417205814422265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT24900.042.5324842
TCGTATG25850.042.17074243
ACGTCTG33050.039.4534915
TATGCCG30200.036.32531446
AGTCACG32750.035.3915128
CACGTCT39550.033.66665314
ACACGTC40750.032.42106213
CGTATGC33600.032.23907544
GTATGCC38850.028.85931645
CGCTATG40050.028.42392529
GGTAGCA41850.027.77800634
ATGCCGT40200.027.71862447
CAGTCAC42650.027.6616627
CTATAAG59350.026.97034346
TAGCATC40550.026.88226536
GTCGCTA42800.026.51693227
GTCTGAA50900.025.9569517
TATAAGC62450.025.6871447
CTTAAAC33350.025.6593671
GTCACGG48000.025.51289629
Produced by FastQC (version 0.11.2)