FastQCFastQC Report
Wed 7 Mar 2018
HYJGYAFXX_n01_ah9082-input-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah9082-input-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5447473
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG18550.015.58209432-33
TGCCGTC21100.013.69895250-51
CCGCACA21250.013.49166436-37
CGTATGC23300.012.30462946-47
TATGCCG22750.012.1889248-49
GTCCGCA23400.012.05119334-35
AGTCACG24200.011.65280628-29
TCACGTC25900.011.43234730-31
GCACATC26600.010.68977138-39
CTCGTAT27100.010.66597344-45
CCGTCTT29050.09.86911852-53
ATCTCGT29500.09.3202742-43
AAGGGGG21750.09.07579474-75
CCAGTCA33100.08.66156726-27
AGGGGGG21550.08.50573674-75
GCCGTCT20400.08.17886150-51
ACATCTC35050.08.11263640-41
CGTCTGA36850.08.0989916-17
CACGTCT37500.08.0839414-15
CGTCCGC19800.08.07064732-33
Produced by FastQC (version 0.11.2)