FastQCFastQC Report
Wed 7 Mar 2018
HYJGYAFXX_n01_ah9082-flag-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah9082-flag-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6436814
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA650391.0104222368395297TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT459890.7144683689788146TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC155450.038.57929250-51
TATGCCG155550.038.5242848-49
CGTATGC156500.038.29042446-47
CGATCTC150200.037.7686740-41
CCGTCTT158750.037.70331652-53
CTCGTAT155450.037.56643744-45
ATCTCGT153850.037.08647542-43
AGATCGG189950.036.757861
AGTCACG166900.035.9190828-29
AACGATC161050.035.4138738-39
TCACGTG171550.034.95916430-31
TGCTTGA172650.034.46366560-61
CCAGTCA175950.034.0315126-27
ACGTGAA177000.033.98842632-33
AAGGGGG128300.033.37229574-75
CTTCTGC181450.033.0512656-57
GTGAAAC180450.033.03907834-35
GAAACGA178150.032.99075336-37
GTCTTCT181700.032.88938554-55
AGAGCAC212950.032.7308929
Produced by FastQC (version 0.11.2)