FastQCFastQC Report
Wed 7 Mar 2018
HYJGYAFXX_n01_ah8867-input-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah8867-input-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9216129
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA123660.13417780936009033TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG52950.024.09897674-75
CCAGTCA73450.022.87606626-27
AGTCACA75650.022.45931228-29
TCACATG77100.022.09788730-31
TGCCGTC39200.021.64142650-51
CGTCTGA79900.021.32343316-17
AGAGCAC110650.021.068089
ACATGTC80500.020.8433332-33
CGTATGC48300.020.67790646-47
CACGTCT83450.020.3881114-15
ATGTCAG83700.020.2149134-35
CACACGT84200.020.0669612-13
TATGCCG47950.020.04469548-49
CTCCAGT85800.019.63802524-25
ATCTCGT58200.018.93715142-43
CTCGTAT52900.018.56886544-45
AGATCGG127950.018.0804021
GATCGGA130750.017.6502572
GTCAGAA96450.017.61573836-37
TGAACTC97500.017.52253320-21
Produced by FastQC (version 0.11.2)