FastQCFastQC Report
Wed 7 Mar 2018
HYJGYAFXX_n01_ah8867-ha-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah8867-ha-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8229982
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA273000.3317139697267868TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT141720.17219964758124623TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGA73100.031.15096936-37
CCCGATC70700.030.41353238-39
TGCCGTC81600.029.77800450-51
CGTATGC84150.028.7918646-47
CCGTCCC85900.028.06850834-35
TATGCCG86500.028.06398848-49
CGATCTC77300.027.69517140-41
CTCGTAT83700.027.6832244-45
ACCCGTC88200.027.33656332-33
CCGTCTT90550.026.88663752-53
TCACCCG91700.026.44710530-31
AAGGGGG63550.026.14382274-75
ATCTCGT85650.025.2146742-43
AGTCACC107750.022.7257528-29
CCAGTCA108250.022.59907326-27
TGCTTGA108950.022.17333860-61
GCCGTCT77500.021.07406252-53
CGTCCCG78500.020.62598836-37
CTTCTGC120050.020.49508356-57
AGGGGGG63300.020.41851474-75
Produced by FastQC (version 0.11.2)