FastQCFastQC Report
Wed 7 Mar 2018
HYJGYAFXX_n01_ah8643-input-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah8643-input-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6956477
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC23700.015.07158950-51
TATGCCG27550.013.05069548-49
CGTATGC29750.012.08560246-47
CTCGTAT33200.010.900544-45
CCGTCTT33650.010.89440152-53
GCCGTCT22800.010.71919852-53
AAGGGGG26800.010.60997674-75
GTATGCC26550.09.82476748-49
AGTCACA38550.09.69265628-29
CCAGTCA38550.09.63169626-27
ATCTCGT36500.09.52867842-43
CGTCTGA39200.09.29210616-17
AGAGCAC59300.09.1938449
TCACAGT43000.09.07213930-31
AGTCAAC42200.08.96555634-35
CACGTCT41850.08.81599114-15
ACAGTCA44100.08.73914732-33
ATGCCGT29850.08.65988550-51
CACACGT41650.08.57621212-13
CTCCAGT48750.08.19485424-25
Produced by FastQC (version 0.11.2)