FastQCFastQC Report
Wed 7 Mar 2018
HYJGYAFXX_n01_ah8643-ha-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah8643-ha-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9915684
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT978540.9868608156532621TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA556330.5610606388828042TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC192150.039.4782750-51
TATGCCG196600.039.1225848-49
CGTATGC198650.038.57689746-47
CTCGTAT198450.037.7868544-45
CCGTATC213200.037.38812338-39
CCGTCTT193200.037.30539752-53
ATCTCGT204950.036.7145742-43
GTATCTC216500.035.0489640-41
TTCCGTA233650.034.45772636-37
GAGCACA310700.034.2929889
AGTCACA239000.034.1685328-29
CCAGTCA241750.033.6340426-27
TCACAGT242750.033.41803730-31
AGAGCAC319650.033.33288
ACAGTTC244950.033.16552732-33
AGTTCCG251150.032.1690134-35
TGCTTGA218050.032.08394660-61
AAGGGGG175550.031.9399874-75
CTTCTGC222700.031.73059756-57
CTCCAGT257100.031.68062224-25
Produced by FastQC (version 0.11.2)