FastQCFastQC Report
Wed 7 Mar 2018
HYJGYAFXX_n01_ah7797-sir4-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah7797-sir4-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5696037
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC768911.3499034504164913TruSeq Adapter, Index 12 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG522850.9179188969453675TruSeq Adapter, Index 12 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG154050.035.07039672-73
TGCCGTC193000.034.8721848-49
CCGTCTT188750.034.84811850-51
TATGCCG196100.034.80025546-47
CTCGTAT185900.034.58598342-43
CGTATGC197700.034.50672544-45
ATCTCGT189150.033.9171840-41
TGCTTGA193050.033.268558-59
GTCTTCT198100.032.59835452-53
CTTCTGC200750.032.285154-55
TCTGCTT202350.031.7743256-57
TAATCTC203500.031.652538-39
TGTAATC206500.031.63647836-37
GAGCACA273300.030.8686929
AGAGCAC277850.030.3801088
AGTCACC236500.029.80994228-29
CCAGTCA239400.029.4390226-27
ACCTTGT241650.029.13522732-33
GATCGGA289000.028.882711
TCGGAAG294550.028.7865723
Produced by FastQC (version 0.11.2)