FastQCFastQC Report
Wed 7 Mar 2018
HYJGYAFXX_n01_ah7797-reson-input-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah7797-reson-input-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7122399
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG18500.013.71880332-33
CGTACGT21850.013.65892434-35
TGCCGTC23600.012.74565550-51
AGGGGGG20700.012.48781774-75
CGTATGC25650.012.09346746-47
GCCGTCT22600.011.54193950-51
TATGCCG27600.010.89845948-49
GAGCACA46500.010.7138899
CTCGTAT29300.010.3463344-45
ACCGTAC31950.09.85594332-33
AGAGCAC51800.09.7991468
GTATGCC27150.09.60765546-47
CACACGT33700.09.27440312-13
CGTAATC32950.09.27154638-39
CCAGTCA35500.09.20133126-27
GATCGGA54400.09.0760761
CCGTCTT34450.09.07249452-53
CACGTCT33950.09.06767114-15
ATCTCGT34400.09.01736642-43
GTACGTA30050.08.83686434-35