FastQCFastQC Report
Wed 7 Mar 2018
HYJGYAFXX_n01_ah7797-reson-fpr3-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah7797-reson-fpr3-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7339395
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT139900.1906151665089561TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGTG39300.026.9081532-33
AGTCACG39400.026.66110428-29
TGCCGTC41950.025.1523350-51
TCACGAG43700.024.30657830-31
GAGCACA62750.023.293819
CGTATGC48050.022.0570346-47
TATGCCG48350.021.7743648-49
AGAGCAC67350.021.7726298
AGGGGGG35750.021.75784174-75
GATCGGA66700.021.7135981
CTCGTAT50650.021.20316344-45
CACACGT50450.020.91457412-13
CACGTCT51500.020.53379214-15
ATCTCGT51750.020.43459542-43
CGTCTGA51750.020.38925416-17
CCGTCTT54150.019.8326952-53
CCAGTCA54850.019.75109326-27
TCGGAAG74850.019.6548653
CACGAGT37200.019.45694230-31
GAGTGGA55450.019.36772234-35
Produced by FastQC (version 0.11.2)