FastQCFastQC Report
Wed 7 Mar 2018
HYJGYAFXX_n01_ah7797-red1-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah7797-red1-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6445617
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC22350.017.45400848-49
AAGGGGG26300.015.81547172-73
CGTATGC27500.015.63806444-45
TATGCCG27600.015.49626146-47
CACGTCT35600.015.1164614-15
AGATCGG43800.014.3854711
CACACGT36600.014.25399112-13
CGTCTGA37700.014.14981716-17
ATCTCGT29800.013.8790940-41
AGGGGGG20200.013.72761874-75
GATCGGA47050.013.5860782
CTCGTAT30050.013.37261142-43
AGTCACC39500.013.20759728-29
CCAGTCA40800.012.95956126-27
AGCACAC43650.012.43633410-11
AGAGCAC52250.012.4133039
CTCCAGT44750.012.02565424-25
TCGGAAG56800.011.3364464
CCGTCTT29100.011.30575750-51
CATCTCG24250.010.7566240-41
Produced by FastQC (version 0.11.2)