FastQCFastQC Report
Wed 7 Mar 2018
HYJGYAFXX_n01_ah7797-input-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah7797-input-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6401498
Sequences flagged as poor quality0
Sequence length100
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC25000.015.97989448-49
TATGCCG27650.014.70333846-47
AAGGGGG26050.014.43368672-73
CGTATGC29400.014.22779744-45
CGATGTA33450.013.91022234-35
CCAGTCA36750.013.36464226-27
AGTCACC36250.013.22484328-29
CACGTCT36250.013.2247414-15
CACACGT35750.013.21249912-13
ACCGATG36650.013.08040332-33
CGTCTGA37550.012.766891516-17
CTCGTAT32950.012.48094842-43
ATCTCGT32300.012.3683440-41
CCGTCTT31700.012.30591250-51
TCACCGA39650.011.97227230-31
AGCACAC44450.010.89081710-11
GAGCACA46000.010.8303629
CTCCAGT45200.010.71014724-25
AGATCGG46100.010.4033691
AGAGCAC48450.010.3797048
Produced by FastQC (version 0.11.2)