FastQCFastQC Report
Wed 7 Mar 2018
HYJGYAFXX_n01_ah7797-hed1-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah7797-hed1-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4601156
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG454210.9871649646306275TruSeq Adapter, Index 5 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC446070.9694737583337754TruSeq Adapter, Index 5 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG162560.3533025178889827TruSeq Adapter, Index 5 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC149680.3253095526428576TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG124800.057.613261
CGTATGC157250.041.5000644-45
TGCCGTC153800.040.97943548-49
TATGCCG159400.040.91081646-47
CCGTCTT147850.040.2444450-51
ACACAGT180900.039.80323832-33
CCAGTCA182450.039.38759626-27
AGTCACA183550.039.305428-29
CTCGTAT118100.039.29892742-43
CGTCTGA185600.038.8961616-17
AAGGGGG146650.038.8591472-73
CACGTCT185700.038.8372514-15
TCACACA186450.038.70665430-31
CACACGT187650.038.48375712-13
AGTGATC127850.038.3054436-37
ATCTCGT120850.038.1129840-41
CTCCAGT190350.037.75270524-25
AGCACAC193300.037.49263810-11
GAGCACA195650.037.4747059
TGATCTC125150.037.32922738-39
Produced by FastQC (version 0.11.2)