FastQCFastQC Report
Wed 7 Mar 2018
HYJGYAFXX_n01_ah7797-fpr3-0318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah7797-fpr3-0318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7185240
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG273110.38009864667011817TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG73300.039.006021
GATCGGA76450.037.319922
AGAGCAC79750.035.7137229
TCGGAAG87250.033.0770154
ATCGGAA98050.029.6254023
CGGAAGA100100.028.7369565
TGCCGTC58400.028.36870448-49
ACTGACC59350.027.91461232-33
CGTATGC60050.027.58921444-45
TATGCCG60550.027.2837746-47
AAGGGGG52300.026.19570272-73
GAAGAGC112350.025.8110267
CTCGTAT62400.025.6840242-43
GCCGTCT55700.025.06077450-51
CGTCTGA66400.025.05704516-17
CACGTCT66600.024.98171414-15
ATCTCGT63650.024.84733840-41
CACACGT67550.024.76953512-13
CCGTCTT66200.024.70668450-51
CCAGTCA69250.024.02590426-27
Produced by FastQC (version 0.11.2)