FastQCFastQC Report
Wed 7 Mar 2018
HYJGYAFXX_n01_ah2243_10nm_estradiol_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah2243_10nm_estradiol_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9466591
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG462170.48821164873395284TruSeq Adapter, Index 9 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC337170.3561683397962371TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC117650.034.71539748-49
CGTATGC125100.032.77950744-45
TATGCCG125900.032.7205446-47
CTCGTAT130400.030.9065742-43
CCGTCTT134000.030.42698750-51
AAGGGGG103100.030.15542872-73
ATCTCGT133650.029.78576340-41
AGATCTC140400.028.27006738-39
AGTCACG153900.026.6454728-29
TGCTTGA159000.025.74632858-59
TCACGAT161550.025.55826830-31
ACGATCA168200.024.49177232-33
CTTCTGC168550.024.39908854-55
GATCAGA172500.023.69052934-35
GTCTTCT174350.023.5604652-53
TCAGATC173400.023.02547336-37
CCAGTCA179300.023.01499226-27
TCTGCTT184200.022.4536756-57
AGGGGGG93950.021.18609472-73
GCCGTCT114100.020.92533350-51
Produced by FastQC (version 0.11.2)