FastQCFastQC Report
Wed 7 Mar 2018
HYJGYAFXX_n01_ah2243_10nm_estradiol_gal4_chip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah2243_10nm_estradiol_gal4_chip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6449541
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG195990.30388209021386176TruSeq Adapter, Index 8 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC118580.18385804509189102TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG96150.040.3457151
TGCCGTC47450.033.87536248-49
CCAGTCA92000.033.8707526-27
AGTCACA93600.033.41742728-29
AGAGCAC115850.033.3076939
GATCGGA119250.032.438122
CGTATGC56150.032.43515844-45
CACGTCT96800.032.3364914-15
CACACGT97650.031.91087312-13
CGTCTGA98350.031.77908116-17
TCACACT99700.031.4199730-31
ACACTTG100200.031.21603832-33
TCGGAAG125550.030.9224744
AGCACAC103300.030.25650210-11
CTCCAGT105200.030.06734324-25
TATGCCG62550.029.86784446-47
CGGAAGA133750.028.9915245
ATCGGAA134550.028.9239443
AAGGGGG81750.028.7746772-73
TGAACTC109350.028.68973520-21
Produced by FastQC (version 0.11.2)