FastQCFastQC Report
Wed 7 Mar 2018
HYJGYAFXX_n01_ah2243_100nm_estradiol_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah2243_100nm_estradiol_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7236390
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC231430.31981416148107TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA80850.031.914659
TCACGGC47350.029.72881730-31
AGAGCAC87750.029.2979988
TGCCGTC49450.028.51344748-49
AGTCACG51750.027.51902428-29
AGGGGGG39800.027.3967972-73
ACGGCTA52450.027.01715532-33
GTCACGG47950.026.95534328-29
GATCGGA95650.026.740761
CACGGCT48000.026.73142830-31
GCCGTCT47800.026.59709248-49
CGTATGC53500.026.39887644-45
CGGCTAC50150.025.49151832-33
TCGGAAG101250.025.4849683
GGCTACA55650.025.33674634-35
CATCTCG49900.025.28939838-39
TATGCCG56700.025.0333346-47
GTATGCC53050.024.40793244-45
CTCGTAT58000.024.10758642-43
CGGAAGA110850.023.362684
Produced by FastQC (version 0.11.2)