FastQCFastQC Report
Wed 7 Mar 2018
HYJGYAFXX_n01_ah2243_100nm_estradiol_gal4_chip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah2243_100nm_estradiol_gal4_chip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6406489
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC26950.020.0554648-49
CACGTCT41450.017.97207614-15
CACACGT41850.017.96875812-13
CGTATGC30600.017.89361444-45
CCAGTCA42850.017.82390626-27
TCACTAG43350.017.72674630-31
TATGCCG32850.017.45492646-47
TAGCTTA43450.017.36116234-35
CGTCTGA44100.017.26520316-17
AGTCACT44700.017.19137428-29
GCTTATC45250.017.03408436-37
ACTAGCT45100.016.98652832-33
AAGGGGG37050.016.110572-73
AGCACAC48000.015.86234410-11
AGATCGG56250.015.3813931
AGAGCAC57850.015.1113469
CTCGTAT35400.015.0690642-43
GATCGGA57350.014.9163322
ATCTCGT37550.014.76949440-41
CTCCAGT54700.013.91953324-25
Produced by FastQC (version 0.11.2)