FastQCFastQC Report
Tue 6 Mar 2018
HYJGYAFXX_n01_ah2243_1000nm_estradiol_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah2243_1000nm_estradiol_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7562685
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG37350.017.86866474-75
AGGGGGG29900.017.6052174-75
AGTCACG49900.017.3777328-29
GTTTCGG49200.017.19498434-35
CGTTTCG47650.013.71036932-33
CGGAATC64300.013.15696938-39
CCAGTCA67150.013.05354926-27
TCACGTT70500.012.53331530-31
ACGTTTC71100.012.361443532-33
GTCACGT52700.012.26279128-29
TATGCCG34500.012.26077148-49
TTCGGAA72000.012.17420736-37
GAGCACA109450.012.1524829
AAAGGGG46200.011.54644372-73
CGTATGC36200.011.42532246-47
AGAGCAC116750.011.3121158
CTCCAGT79350.011.22423224-25
TGCCGTC28500.011.04903350-51
CGTCTGA80300.010.82805616-17
CTCGTAT37150.010.69035744-45
Produced by FastQC (version 0.11.2)