FastQCFastQC Report
Tue 6 Mar 2018
HYJGYAFXX_n01_ah2243_1000nm_estradiol_gal4_chip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah2243_1000nm_estradiol_gal4_chip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6629337
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT160880.24267886818847795TruSeq Adapter, Index 20 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTA147970.2232048242531644TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG49150.031.79526728-29
TGCCGTC48500.031.34923250-51
ACGTGGC51600.030.05789632-33
TCACGTG52600.029.84386330-31
GGCCTTA53000.029.08655536-37
CGTATGC54200.028.44257446-47
TATGCCG57500.026.89195448-49
CTCGTAT58700.025.86180144-45
CACACGT62250.025.33071912-13
CACGTCT62400.025.19450814-15
CCAGTCA62800.025.07145526-27
CCGTCTT61850.024.84862552-53
AGATCGG61250.024.799321
ATCTCGT61850.024.65865142-43
CGTCTGA63850.024.62235316-17
GTGGCCT64500.024.22848534-35
AGCACAC68800.022.95330210-11
GAGCACA71100.022.8718269
CTCCAGT72700.021.8835924-25
AAGGGGG52550.021.15206574-75
Produced by FastQC (version 0.11.2)