FastQCFastQC Report
Tue 6 Mar 2018
HYJGYAFXX_n01_ah2243_0nm_estradiol_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah2243_0nm_estradiol_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8258809
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC243260.2945461022285417TruSeq Adapter, Index 3 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG176500.21371120218423748TruSeq Adapter, Index 3 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC66500.031.38020948-49
CGTATGC75600.028.90850844-45
TATGCCG76800.028.45681246-47
ACTTAGG82900.028.37553632-33
AAGGGGG67500.027.67756572-73
CTCGTAT77300.026.69190242-43
TTAGGCA88250.026.6020634-35
AGGCATC84600.026.5553536-37
GCATCTC78350.026.51415438-39
ATCTCGT80450.025.64678840-41
CCGTCTT80050.025.2758850-51
CCAGTCA106250.022.36076726-27
AGTCACT107100.022.05165128-29
AGGGGGG60250.020.75004872-73
TCACTTA116700.020.49941330-31
TGCTTGA102050.019.57358758-59
CTCCAGT122850.019.28190224-25
CTTCTGC109350.018.2024254-55
CGTCTGA130900.017.9704516-17
GCCGTCT63650.017.758748-49
Produced by FastQC (version 0.11.2)