FastQCFastQC Report
Tue 6 Mar 2018
HYJGYAFXX_n01_ah2243_0nm_estradiol_gal4_chip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJGYAFXX_n01_ah2243_0nm_estradiol_gal4_chip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7823780
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC294600.3765443302342346TruSeq Adapter, Index 1 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG268990.3438107922257528TruSeq Adapter, Index 1 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC85300.034.8501848-49
CGATCTC81900.034.0875738-39
CGTATGC89250.033.3077944-45
CACGATC84250.033.0251936-37
TATGCCG92550.032.34867546-47
CTCGTAT93400.031.04786942-43
CCGTCTT98200.030.29603250-51
ATCTCGT93800.029.93840240-41
ATCACGA101500.028.6627734-35
AAGGGGG81150.028.2055172-73
AGTCACA109750.027.1938728-29
CCAGTCA111300.026.8151626-27
TCACATC117050.025.45773130-31
ACATCAC123350.024.48090632-33
TGCTTGA122550.024.31473258-59
CTCCAGT128150.023.19741624-25
AGGGGGG72750.022.93447972-73
CTTCTGC131350.022.9004254-55
CGTCTGA133100.022.58174116-17
GTCTTCT137800.022.0672752-53
Produced by FastQC (version 0.11.2)