FastQCFastQC Report
Wed 9 Mar 2016
HYJ7LBGXX_n01_bzip1ko2_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJ7LBGXX_n01_bzip1ko2_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18526253
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC734030.39621071783916584No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG510730.27567905933272097No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT470400.2539099514618525No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG432840.23363601911298523No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC275660.148794254294163No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA258220.13938058602567935No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC253660.1369192140472226No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA249630.1347439225838058No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG235460.12709531711566283No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC215300.11621346205301201No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA206750.11159838959340564No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC200790.10838133323559816No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT199850.1078739451523198No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT197620.10667024789092537No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA196220.10591456351157463No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC195250.10539098219159589No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT193910.10466768428564589No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC193040.10419808042133508No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT192640.10398217059866342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT99300.035.508611
CTAGCAG126650.028.5738228
CTGACTC128000.027.9489442
ACTCTAG134600.027.2291645
GACTCTA144100.025.7253424
TAGCAGA150850.023.9203479
TGACTCT156700.023.3885543
CTCTAGC165100.022.580716
CTATCGA165600.021.64152717
TCTATCG173300.020.55889716
GATCTAT184900.019.32594114
ATCTATC187150.019.168415
GCAGATC189300.018.93245711
AGCAGAT198850.018.25191710
AGATCTA204850.017.56336813
TAGCATG203000.017.53685627
TATCGAT205650.017.49471318
CTAGCAT207900.017.19078626
GCATGAC204900.017.18617829
GTGCGCA187650.016.8602368
Produced by FastQC (version 0.11.2)