FastQCFastQC Report
Wed 9 Mar 2016
HYJ7LBGXX_n01_bzip1ko2_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJ7LBGXX_n01_bzip1ko2_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27286780
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC1193020.4372153841530588No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG954670.34986539269199224No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG617700.22637335735473368No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT613580.22486346868336973No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA400320.14670840604864333No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA394470.1445645107264397No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC354840.1300409942103832No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC351540.1288316173619606No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA340240.12469041785069547No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG329750.12084606538404312No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC300790.11023286734455293No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT287390.10532206438429159No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA284550.10428126733898248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT157650.029.138731
CTAGCAG189950.024.8187988
CTGACTC189250.024.3406032
ACTCTAG198950.024.0868725
GACTCTA204550.023.7181844
TAGCAGA225650.021.0163829
TGACTCT231900.020.6036453
CTCTAGC242750.019.7699096
CTATCGA242500.018.97880617
TCTATCG252100.018.27000616
GTGCGCA257000.017.79867768
TGCGCAT264550.017.35686769
GATCTAT276750.016.74375314
GCAGATC281650.016.58941711
GGTGCGC280700.016.52039167
GCGCTTC538150.016.4926868
ATCTATC282950.016.45123115
AGCAGAT289300.016.40464610
CGCTTCA548450.016.20846769
AGATCTA294850.015.83463113
Produced by FastQC (version 0.11.2)