FastQCFastQC Report
Wed 9 Mar 2016
HYJ7LBGXX_n01_bzip1ko2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJ7LBGXX_n01_bzip1ko2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29467894
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC1415460.48033972159666377No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG1066320.3618582311990127No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT815170.27662988064230176No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG681260.23118720326603592No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA467610.158684567007062No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA444490.1508387399520305No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC437950.14861937537850514No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC423290.14364446946904316No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG401810.13635518031929936No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA366490.12436925421273742No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA355540.12065334563779821No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT353200.11985926106561941No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC334520.11352015858344No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC309060.10488024695622972No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC302450.10263712771601526No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT300280.1019007330486529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT169450.035.6444631
CTAGCAG215550.028.4793518
CTGACTC211900.028.4787642
ACTCTAG227050.026.9950355
GACTCTA238600.026.2015724
TGACTCT252300.024.3901793
TAGCAGA254850.024.1015769
CTCTAGC273550.022.5598566
CTATCGA282850.021.51812617
TCTATCG293250.020.69535416
GATCTAT314600.019.31310314
ATCTATC323050.018.76481815
GCAGATC329350.018.63947711
AGCAGAT337500.018.36538110
AGATCTA343600.017.79505313
TATCGAT347350.017.54212418
TAGCATG355850.017.03719127
GCATGAC354150.016.95071629
CTAGCAT362550.016.76084726
TTCTAGC363600.016.65377824
Produced by FastQC (version 0.11.2)