FastQCFastQC Report
Wed 9 Mar 2016
HYJ7LBGXX_n01_bzip1ko2_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJ7LBGXX_n01_bzip1ko2_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13949185
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC708410.5078504586468672No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG500050.3584797248011264No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG411300.2948559360278038No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT379480.27204456747831507No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA310620.22267967626782495No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA236880.16981637278450318No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC205740.14749248791237624No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA203280.14572894402074388No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG196400.1407967562262598No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC196030.14053150775475415No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC176390.12645183213212816No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT171810.12316848618754431No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC168910.12108951168114838No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC168420.12073823667834357No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT163910.11750507287701754No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA163210.11700325144443924No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT158560.11366972335659753No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTG149980.10751882636870899No Hit
CAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC147130.10547569625035441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT84600.033.234621
CTGACTC102350.027.3940542
CTAGCAG104050.027.3462528
ACTCTAG113750.025.4797555
GACTCTA118800.024.3670124
TAGCAGA121250.023.6117789
TGACTCT127450.022.4934313
CTCTAGC134100.021.7177856
CTATCGA140000.020.14942217
TCTATCG142800.019.70531716
GATCTAT154500.018.30387914
GTGCGCA159650.018.19501168
AGCAGAT159950.018.008310
TGCGCAT163300.017.80975569
ATCTATC158650.017.80314815
GCAGATC159350.017.68095211
GGTGCGC169800.017.18982767
AGATCTA165600.017.09806313
TATCGAT169400.016.75547818
TCTAGCA273400.016.4879517
Produced by FastQC (version 0.11.2)