FastQCFastQC Report
Wed 9 Mar 2016
HYJ7LBGXX_n01_bzip1ko2_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJ7LBGXX_n01_bzip1ko2_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20218659
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC826830.4089440353091667No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG584800.28923777783679916No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT507850.2511788739302641No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG472760.23382361807476945No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA312400.15451074178559518No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA272530.13479133309484076No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA270200.13363893223581247No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG259000.12809949463018294No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC251380.12433069868778143No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC242040.11971120339880108No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT241990.11968647376663309No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCT223800.1106898335839187No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC221250.10942862234335127No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA217080.10736617102054097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT112250.031.378721
CTAGCAG141000.025.2190328
CTGACTC143100.024.8273092
ACTCTAG149650.024.6765445
GACTCTA158400.023.6226124
TGACTCT172800.020.9245723
TAGCAGA172400.020.7681399
CTCTAGC186550.019.908156
CTATCGA189100.018.80420317
TCTATCG196800.018.0683816
GTGCGCA209400.017.2652268
TGCGCAT215300.016.8733769
GATCTAT212250.016.75332314
ATCTATC214600.016.74930215
GGTGCGC222050.016.37616367
GCAGATC217750.016.3143711
TCTAGCA365250.015.8872687
AGCAGAT229200.015.65198910
TATCGAT230700.015.38284718
AGGTGCG238050.015.29010266
Produced by FastQC (version 0.11.2)