FastQCFastQC Report
Wed 9 Mar 2016
HYJ7LBGXX_n01_bzip1ko1_9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJ7LBGXX_n01_bzip1ko1_9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18427197
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC653090.3544163553469364No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG557170.30236286072157365No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT455230.2470424557788143No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG362560.1967526585839398No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA347700.18868849125561526No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC261700.14201834386423504No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG261490.14190438187641888No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC255310.138550643377829No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA253210.13741102349966736No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA219750.1192530801076257No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA218350.11849333352218464No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC201110.10913759699861027No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC192190.10429692589708571No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC189010.10257121579586954No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT187120.1015455579055241No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT103600.032.4117471
CTGACTC129450.026.1210942
CTAGCAG134150.025.5411878
ACTCTAG145500.024.2982965
GACTCTA149300.023.8672874
TGACTCT156550.021.822753
TAGCAGA162700.021.2961679
CTCTAGC176400.020.3397256
CTATCGA176550.019.2093317
TCTATCG182150.018.4457316
GTGCGCA151300.017.44145468
GATCTAT196800.017.12605714
ATCTATC198650.017.05470315
GCAGATC204450.016.77653311
TGCGCAT158300.016.73638569
GGTGCGC162050.016.37082167
AGCAGAT215000.016.14835210
AGATCTA218200.015.67097313
TAGCATG217100.015.60710527
CTAGCAT219500.015.53209726
Produced by FastQC (version 0.11.2)