FastQCFastQC Report
Wed 9 Mar 2016
HYJ7LBGXX_n01_bzip1ko1_8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJ7LBGXX_n01_bzip1ko1_8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61631780
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC1998320.32423532145266615No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG1609790.26119479268650037No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT1586690.25744672634799776No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG1197150.19424232108824377No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA1128690.18313441539413594No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG838440.1360402052317814No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC822620.13347334767874627No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC813290.1319595182874809No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA764550.12405126056719439No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA748440.12143734936748542No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA697850.11322892183221059No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAAC678010.11000980338390356No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCT624400.10131136890740458No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC619810.10056662325832548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT346300.033.7402041
CTGACTC437850.026.8136652
CTAGCAG441950.026.6164478
ACTCTAG480200.025.0762255
GACTCTA498800.024.5620144
TAGCAGA534450.022.1540499
TGACTCT542250.022.0708163
CTCTAGC581950.020.830256
CTATCGA591700.019.9155717
TCTATCG609250.019.11781716
GTGCGCA490250.017.81144768
GATCTAT668700.017.4863514
ATCTATC676900.017.33137915
GCAGATC688000.017.07741711
TGCGCAT514250.017.07545569
AGCAGAT703300.016.93970110
GGTGCGC528850.016.5908767
AGATCTA719850.016.36536613
TAGCATG717250.016.25627927
GCATGAC714450.016.18758629
Produced by FastQC (version 0.11.2)