FastQCFastQC Report
Wed 9 Mar 2016
HYJ7LBGXX_n01_bzip1ko1_7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJ7LBGXX_n01_bzip1ko1_7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26726742
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC1050160.39292480916678885No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAG976040.3651922856889927No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT559210.20923238604989713No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGG514130.19236538445277018No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA436450.16330086173615924No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGC434810.1626872441092895No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGA425440.15918139218016172No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC405650.15177682337787374No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTG321250.12019796501945505No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAT304350.11387471020598021No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTC287970.10774601707907384No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCC287900.107719826082805No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGA285950.10699021975817329No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGA276860.10358913181412087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT136800.028.7435041
CTAGCAG171600.023.3937348
CTGACTC174250.022.6996942
ACTCTAG191100.021.284725
GACTCTA199700.020.9814414
TAGCAGA208950.019.296059
TGACTCT215300.018.6154773
CTATCGA222950.017.73897217
CTCTAGC232500.017.6603766
TCTATCG234050.016.8229516
GTGCGCA213900.016.34553768
GCGCTTC476800.016.12643268
CGCTTCA488150.015.83750969
TGCGCAT221500.015.80049669
GATCTAT254800.015.42562314
GGTGCGC231600.015.1266167
GCAGATC265200.015.07153111
ATCTATC262100.015.0494315
TCTAGCA428650.014.5579537
AGCAGAT280650.014.44115110
Produced by FastQC (version 0.11.2)