FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29718399
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1895740.637901119774319No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1441610.4850900615473936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG253300.036.7196163
CGGAAGA257950.036.3275034
ATCGGAA263550.035.3258132
GATCGGA263000.034.6527141
GTAGGGA171750.026.54892518-19
TCGTGTA172400.026.45730614-15
TAGGGAA173650.025.21958218-19
GTGTAGG185600.024.5457616-17
TGTAGGG178600.024.40454316-17
AAAAGGG299900.024.38937680-81
GAGCGTC375400.024.170569
AGCGTCG191450.023.9600410-11
AAGGGGG311950.022.98512882-83
AGAGCGT406150.022.3885428
AAGAGCG423850.022.0767847
ATTACGA28750.021.07128554-55
TAGGGGG52550.020.75287250-51
TATTACG22550.020.64896454-55
AGGGAAA233550.020.5615420-21
TTAAGGG49150.020.24630448-49
Produced by FastQC (version 0.11.5)