FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n02_HFFLm28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n02_HFFLm28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22549993
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG878300.027.31002480-81
AAGGGGG957750.025.02072782-83
TAGGGGG171950.022.22214150-51
CGGGTTT93700.021.99924940-41
TAAGGGG198600.021.73935550-51
GGGTTTT450100.020.65606542-43
AAAAAAG1283150.020.20883878-79
AACGGGT39300.019.63893938-39
GGGAAAA1073500.019.33212922-23
GGGGAAA1165150.018.75921822-23
TTGGGGG624250.018.66764650-51
ACGGGTT55950.018.633440-41
GGGAATA171550.018.61937164-65
GGTTTTT533250.018.29911442-43
TTTTGGG494750.018.07694848-49
AAAGGGG867950.017.91941680-81
AGGGGTT184650.017.88980140-41
GAAAAAG538100.017.87799324-25
TTAAGGG187250.017.78102548-49
GGGGGAA1259500.017.57830620-21
Produced by FastQC (version 0.11.5)