FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n02_HFFLm27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n02_HFFLm27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29775462
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG919950.027.0986980-81
AAGGGGG966600.025.74503582-83
TAGGGGG191200.021.48742550-51
TAAGGGG209400.020.742650-51
GGGAAAA1201400.019.3436122-23
AAAAAAG1411950.019.21139178-79
TTAAGGG209200.018.88946348-49
TTTTGGG670850.018.87878848-49
TTGGGGG729500.018.80954750-51
GGGGAAA1279850.018.20616122-23
GGTTTTT615850.017.86169442-43
GGGAATA205100.017.78485364-65
GGGGGAA1353050.017.46527320-21
AAAGGGG898750.016.8562580-81
GGGGGGA1457600.016.54978420-21
GAAAAAG664600.015.954288524-25
GGGGAAT264100.015.48426262-63
AAAGTTT463300.015.43362728-29
TTTAGGG234900.015.35687148-49
AAAAGTT483850.014.6995826-27
Produced by FastQC (version 0.11.5)