FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n02_HFFLm26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n02_HFFLm26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22431052
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG792900.027.6933380-81
AAGGGGG867350.025.40476482-83
TAAGGGG171800.025.32397350-51
TAGGGGG151350.024.41499950-51
TTAAGGG168000.022.20728748-49
TTTTGGG488850.021.63730448-49
AAAAAAG1140250.020.81894178-79
GGGAAAA1034100.019.89197522-23
GGGAATA162800.019.5614764-65
TTGGGGG588300.019.17047550-51
TTTAGGG166100.018.95843348-49
GGGGAAA1113250.018.50542622-23
GAAAAAG518750.018.38943724-25
GGGGGAA1195500.017.66726520-21
AAAGGGG788900.017.55940280-81
TTTAAGG215200.016.99444648-49
GGGGGGA1314400.016.45212220-21
TTTGGGG539100.015.90257350-51
GGGGAAT225050.015.77579462-63
TTTTTTG714550.015.50411946-47
Produced by FastQC (version 0.11.5)