FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n02_HFFLm25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n02_HFFLm25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23084585
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG777750.028.06485480-81
TAAGGGG181150.026.48165550-51
AAGGGGG846950.025.69147782-83
TTAAGGG172000.023.8173648-49
TAGGGGG148250.022.89125350-51
AAAAAAG1139450.020.80243578-79
TTTTGGG474700.020.05125248-49
GGGAAAA1031700.019.6851122-23
TTGGGGG547600.018.82167650-51
GGGGAAA1097800.018.46090522-23
GGGAATA164100.018.33565164-65
GGGGGAA1156650.017.85628520-21
TTTAAGG224450.017.72263148-49
TTTAGGG164750.017.64367548-49
GAAAAAG528500.017.55581924-25
AAAGGGG764400.017.32997380-81
GGGGGGA1252700.016.75256720-21
AAAGTTT378350.016.67080528-29
AAAAGTT380850.016.33694826-27
TTTGGGG514200.016.12314850-51
Produced by FastQC (version 0.11.5)