FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n02_HFFLm24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n02_HFFLm24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29086818
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG939300.025.78684280-81
AAGGGGG994700.024.49257982-83
TAGGGGG192750.022.22638150-51
GGTTTTT622600.021.42857442-43
TAAGGGG221450.021.35119650-51
TTTTGGG586750.018.86468948-49
AAAAAAG1412750.018.64238478-79
TTGGGGG684350.018.6034850-51
AAAGGGG907350.018.42125980-81
GGGAAAA1135100.018.36254922-23
GGGAATA205050.018.07866564-65
GGGGAAA1230950.018.04008922-23
TTAAGGG225250.017.72256348-49
GGGGGAA1328850.017.0576920-21
GGGGGGA1490950.016.73857720-21
AGGGTTT334100.016.01439740-41
GGGGAAT262350.015.6417762-63
AGGGGTT218650.015.04271340-41
GAAAAAG631950.015.04269724-25
AAAAAGG1143900.015.02677278-79
Produced by FastQC (version 0.11.5)