FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n02_HFFLm23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n02_HFFLm23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22528898
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG737950.025.37303580-81
AAGGGGG779900.024.23709182-83
GGGGTTT420050.023.27595740-41
GGTTTTT492550.022.23638742-43
TAGGGGG168500.020.5770750-51
AAAGGGG724300.019.47366180-81
GGGGGTT358300.018.83670640-41
GGGAATA166350.018.71568164-65
GGGAAAA859950.018.40571622-23
AAAAAAG1106600.018.40511978-79
TTTTGGG494100.018.38436548-49
GGGGAAA949250.018.32787322-23
TTGGGGG632800.017.46212850-51
GGGGGAA1042150.016.96982420-21
TAAGGGG154750.016.8961350-51
GGGGGGA1202300.016.89220-21
GGGGAAT209400.016.39903662-63
AAAAAGG897050.016.29506178-79
GGGTTTT446150.016.25066642-43
GAAAAAG483650.015.51115624-25
Produced by FastQC (version 0.11.5)