FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n02_HFFLm22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n02_HFFLm22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26818620
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG776650.026.05939980-81
AAGGGGG830650.024.40918482-83
TAAGGGG186700.023.21405650-51
TTAAGGG180850.020.90528948-49
TAGGGGG151000.019.89509650-51
TTTTGGG479150.018.96291548-49
GGGAAAA986050.018.85506622-23
AAAAAAG1194700.018.50865278-79
GGGGAAA1063350.017.80822822-23
GGGGGAA1116600.017.25038120-21
TTGGGGG565600.016.94627450-51
AAAGGGG759800.016.63191280-81
GGGGGGA1209250.016.59640120-21
GGGAATA183550.016.52194864-65
TTTAAGG241550.015.6420948-49
GGGGAAT228000.015.4466262-63
AAAGTTT406200.015.27061128-29
TTTGGGG521600.015.12952250-51
GAAAAAG566950.014.9283724-25
TTTAGGG178350.014.43403748-49
Produced by FastQC (version 0.11.5)