FastQCFastQC Report
Thu 28 Feb 2019
HYGYGBGX9_n02_HFFLm21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYGYGBGX9_n02_HFFLm21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24986778
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG311630.12471796083512647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG1338050.030.0398780-81
TAAGGGG244400.029.19958750-51
GGGGTTT611300.028.14585340-41
TAGGGGG252200.027.69385550-51
AAGGGGG1464600.027.523182-83
GGTTTTT881450.027.42430742-43
GGGAATA246250.025.46939764-65
AAAAAAG1747400.024.84249978-79
TTAAGGG243700.024.51817348-49
GGGGAAT305600.023.26626462-63
GAAAAAG704000.022.88406424-25
TTTAGGG244150.021.8467148-49
GGGAAAA1663850.021.54475222-23
GGGGAAA1787500.020.98836722-23
AAGTTTT456750.020.92200528-29
AAAGGGG1327800.020.82268180-81
AAAGTTT459350.020.63297728-29
AAAAGTT469150.020.49552726-27
TTGGGGG1054550.020.3129450-51
AAAAAGT579650.019.90282426-27
Produced by FastQC (version 0.11.5)